Advanced Gut Health & Leaky Gut Screening

Pinpoint gut dysfunction, microbial imbalances, and intestinal permeability — all from one comprehensive test.
The GI-MAP + Zonulin test is one of the most advanced tools for understanding what’s really going on in the gut. From pathogens and parasites to commensal bacteria, fungi, and inflammation markers, this test offers a complete overview of your gastrointestinal ecosystem — and adds critical insight into leaky gut (intestinal permeability) through Zonulin analysis.
 
Why It Matters

Your gut is home to trillions of microbes that help regulate immunity, inflammation, digestion, mood, and more. But when imbalances occur — whether from stress, infections, antibiotics, or diet — the effects can ripple throughout the entire body.
The GI-MAP uses state-of-the-art qPCR DNA technology to accurately identify the DNA of bacteria, viruses, parasites, yeast, and even virulence and antibiotic resistance genes. This allows us to create a precise, personalised healing plan tailored to your microbiome and immune response.
Adding Zonulin testing brings an important extra layer: it helps identify whether the tight junctions of your intestinal lining are being compromised — a key factor in conditions like:
Leaky gut syndrome
Celiac disease
Non-celiac gluten sensitivity (NCGS)
Autoimmune issues
Chronic inflammation
 
Ideal For Those Experiencing:
Bloating, IBS, or irregular bowel movements
Brain fog, skin issues, or fatigue
Suspected leaky gut or gluten sensitivity
Autoimmune flare-ups
A history of food poisoning, travel bugs, or overuse of antibiotics
Persistent infections, dysbiosis, or gut-based inflammation
 
What’s Measured
Pathogenic Microbes
Bacteria: H. pylori (with virulence factors), Salmonella, Campylobacter, E. coli strains, Shigella, and more
Viruses: Norovirus GI/GII, Adenovirus
Parasites & Protozoa: Giardia, Cryptosporidium, Entamoeba histolytica, Blastocystis hominis
Worms: Ascaris, Hookworm, Trichuris, Taenia
Dysbiosis & Microbiome Balance
Commensal bacteria like Akkermansia, Faecalibacterium prausnitzii, Bacteroides, Lactobacillus, and Bifidobacteria
Opportunistic or overgrowth species (Pseudomonas, Klebsiella, Staphylococcus, etc.)
Yeast & fungi including Candida, Geotrichum, and Microsporidia
Bacterial phyla ratios (Firmicutes / Bacteroidetes)
Intestinal Health & Function
Elastase-1 (pancreatic output)
Calprotectin (inflammation)
Beta-glucuronidase, Steatocrit, Occult blood
SIgA & anti-gliadin SIgA (immune defence and gluten sensitivity markers)
Zonulin (Intestinal Permeability Marker)
Zonulin regulates the tight junctions between gut cells. Elevated levels suggest increased permeability — commonly referred to as “leaky gut” — which has been linked to systemic inflammation, autoimmune disease, allergies, and chronic illness.
 
Optional Add-On: Antibiotic Resistance Genes

Want a deeper look at antimicrobial resistance? The GI-MAP can assess genes in Helicobacter pylori for resistance to common antibiotics (e.g. Amoxicillin, Clarithromycin, Fluoroquinolones, Tetracycline).
 
What Makes This Test Different?
Unlike traditional culture-based stool tests, the GI-MAP uses quantitative PCR (qPCR) to detect microbial DNA with high sensitivity and specificity. It not only tells us what’s there but how much, allowing us to prioritise what needs to be treated urgently — and what’s improving over time.
 
The Vitality Project Approach
We combine this cutting-edge diagnostic tool with a personalised wellness strategy to help you:
Rebalance your gut microbiome
Reduce gut inflammation
Repair the intestinal lining
Support immune and metabolic health
Simple at-home stool collection
Guided interpretation + personalised protocol
Track progress with optional retesting
 
If you’re struggling with stubborn symptoms or have been told “everything looks normal” — this test could finally reveal the why.
 
Want to talk it through? Book a free discovery call to explore whether this test is right for you.